Loop-mediated isothermal amplification (LAMP) assay is a novel method of gene amplification that amplifies nucleic acid, which can be applied for disease diagnosis in shrimp aquaculture. During the LAMP reaction, the white precipitate of magnesium pyrophosphate (Mg2P2O7), which indicates the virus in shrimp, is formed correlates with the amount of synthesized DNA. So, the turbidity can be measured by Real-time Turbidimeter. In this work, a Real-time Turbidimeter is developed to detect Shrimp Virus, which caused large economic losses to most major shrimp-producing countries including Thailand.

The principle of Real-time Turbidimeter. The device incorporate a heating block that maintain the optimal temperature of 63 ºC for the duration of the 25 µl shrimp sample perform in a 0.2 ml tube. We also applied the spectroscopic measurement technique to measure a by-product of LAMP reaction. The white turbidity of shrimp sample can be real-time monitored when light from an inexpensive light-emitting diode (LED) pass through the tube to reach a photo diode.

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Nanoelectronics and MEMS Laboratory